Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic.
Identifieur interne : 000475 ( Main/Exploration ); précédent : 000474; suivant : 000476Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic.
Auteurs : Robin Augustine [Qatar] ; Anwarul Hasan [Qatar] ; Suvarthi Das [États-Unis] ; Rashid Ahmed [Qatar] ; Yasuyoshi Mori [Japon] ; Tsugunori Notomi [Japon] ; Bhavesh D. Kevadiya [États-Unis] ; Avnesh S. Thakor [États-Unis]Source :
- Biology [ 2079-7737 ] ; 2020.
Abstract
The rampant spread of COVID-19 and the worldwide prevalence of infected cases demand a rapid, simple, and cost-effective Point of Care Test (PoCT) for the accurate diagnosis of this pandemic. The most common molecular tests approved by regulatory bodies across the world for COVID-19 diagnosis are based on Polymerase Chain Reaction (PCR). While PCR-based tests are highly sensitive, specific, and remarkably reliable, they have many limitations ranging from the requirement of sophisticated laboratories, need of skilled personnel, use of complex protocol, long wait times for results, and an overall high cost per test. These limitations have inspired researchers to search for alternative diagnostic methods that are fast, economical, and executable in low-resource laboratory settings. The discovery of Loop-mediated isothermal Amplification (LAMP) has provided a reliable substitute platform for the accurate detection of low copy number nucleic acids in the diagnosis of several viral diseases, including epidemics like Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). At present, a cocktail of LAMP assay reagents along with reverse transcriptase enzyme (Reverse Transcription LAMP, RT-LAMP) can be a robust solution for the rapid and cost-effective diagnosis for COVID-19, particularly in developing, and low-income countries. In summary, the development of RT-LAMP based diagnostic tools in a paper/strip format or the integration of this method into a microfluidic platform such as a Lab-on-a-chip may revolutionize the concept of PoCT for COVID-19 diagnosis. This review discusses the principle, technology and past research underpinning the success for using this method for diagnosing MERS and SARS, in addition to ongoing research, and the prominent prospect of RT-LAMP in the context of COVID-19 diagnosis.
DOI: 10.3390/biology9080182
PubMed: 32707972
PubMed Central: PMC7464797
Affiliations:
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wicri:level="1"><nlm:affiliation>Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, Doha 2713, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, Doha 2713</wicri:regionArea><wicri:noRegion>Doha 2713</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biomedical Research Center (BRC), Qatar University, Doha PO Box 2713, Qatar.</nlm:affiliation><country xml:lang="fr">Qatar</country><wicri:regionArea>Biomedical Research Center (BRC), Qatar University, Doha PO Box 2713</wicri:regionArea><wicri:noRegion>Doha PO Box 2713</wicri:noRegion></affiliation></author><author><name sortKey="Mori, Yasuyoshi" sort="Mori, Yasuyoshi" uniqKey="Mori Y" first="Yasuyoshi" last="Mori">Yasuyoshi Mori</name><affiliation wicri:level="1"><nlm:affiliation>Eiken Chemical Co., Ltd., Research and Development Division, Taito-ku 110-8408, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Eiken Chemical Co., Ltd., Research and Development Division, Taito-ku 110-8408</wicri:regionArea><wicri:noRegion>Taito-ku 110-8408</wicri:noRegion></affiliation></author><author><name sortKey="Notomi, Tsugunori" sort="Notomi, Tsugunori" uniqKey="Notomi T" first="Tsugunori" last="Notomi">Tsugunori Notomi</name><affiliation wicri:level="1"><nlm:affiliation>Eiken Chemical Co., Ltd., Research and Development Division, Taito-ku 110-8408, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Eiken Chemical Co., Ltd., Research and Development Division, Taito-ku 110-8408</wicri:regionArea><wicri:noRegion>Taito-ku 110-8408</wicri:noRegion></affiliation></author><author><name sortKey="Kevadiya, Bhavesh D" sort="Kevadiya, Bhavesh D" uniqKey="Kevadiya B" first="Bhavesh D" last="Kevadiya">Bhavesh D. Kevadiya</name><affiliation wicri:level="4"><nlm:affiliation>Interventional Regenerative Medicine and Imaging Laboratory, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA 94304, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Interventional Regenerative Medicine and Imaging Laboratory, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA 94304</wicri:regionArea><placeName><region type="state">Californie</region><settlement type="city">Stanford (Californie)</settlement></placeName><orgName type="university">Université Stanford</orgName></affiliation></author><author><name sortKey="Thakor, Avnesh S" sort="Thakor, Avnesh S" uniqKey="Thakor A" first="Avnesh S" last="Thakor">Avnesh S. Thakor</name><affiliation wicri:level="4"><nlm:affiliation>Interventional Regenerative Medicine and Imaging Laboratory, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA 94304, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Interventional Regenerative Medicine and Imaging Laboratory, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA 94304</wicri:regionArea><placeName><region type="state">Californie</region><settlement type="city">Stanford (Californie)</settlement></placeName><orgName type="university">Université Stanford</orgName></affiliation></author></analytic><series><title level="j">Biology</title><idno type="ISSN">2079-7737</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The rampant spread of COVID-19 and the worldwide prevalence of infected cases demand a rapid, simple, and cost-effective Point of Care Test (PoCT) for the accurate diagnosis of this pandemic. The most common molecular tests approved by regulatory bodies across the world for COVID-19 diagnosis are based on Polymerase Chain Reaction (PCR). While PCR-based tests are highly sensitive, specific, and remarkably reliable, they have many limitations ranging from the requirement of sophisticated laboratories, need of skilled personnel, use of complex protocol, long wait times for results, and an overall high cost per test. These limitations have inspired researchers to search for alternative diagnostic methods that are fast, economical, and executable in low-resource laboratory settings. The discovery of Loop-mediated isothermal Amplification (LAMP) has provided a reliable substitute platform for the accurate detection of low copy number nucleic acids in the diagnosis of several viral diseases, including epidemics like Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). At present, a cocktail of LAMP assay reagents along with reverse transcriptase enzyme (Reverse Transcription LAMP, RT-LAMP) can be a robust solution for the rapid and cost-effective diagnosis for COVID-19, particularly in developing, and low-income countries. In summary, the development of RT-LAMP based diagnostic tools in a paper/strip format or the integration of this method into a microfluidic platform such as a Lab-on-a-chip may revolutionize the concept of PoCT for COVID-19 diagnosis. This review discusses the principle, technology and past research underpinning the success for using this method for diagnosing MERS and SARS, in addition to ongoing research, and the prominent prospect of RT-LAMP in the context of COVID-19 diagnosis.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32707972</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2079-7737</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>8</Issue><PubDate><Year>2020</Year><Month>07</Month><Day>22</Day></PubDate></JournalIssue><Title>Biology</Title><ISOAbbreviation>Biology (Basel)</ISOAbbreviation></Journal><ArticleTitle>Loop-Mediated Isothermal Amplification (LAMP): A Rapid, Sensitive, Specific, and Cost-Effective Point-of-Care Test for Coronaviruses in the Context of COVID-19 Pandemic.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E182</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/biology9080182</ELocationID><Abstract><AbstractText>The rampant spread of COVID-19 and the worldwide prevalence of infected cases demand a rapid, simple, and cost-effective Point of Care Test (PoCT) for the accurate diagnosis of this pandemic. The most common molecular tests approved by regulatory bodies across the world for COVID-19 diagnosis are based on Polymerase Chain Reaction (PCR). While PCR-based tests are highly sensitive, specific, and remarkably reliable, they have many limitations ranging from the requirement of sophisticated laboratories, need of skilled personnel, use of complex protocol, long wait times for results, and an overall high cost per test. These limitations have inspired researchers to search for alternative diagnostic methods that are fast, economical, and executable in low-resource laboratory settings. The discovery of Loop-mediated isothermal Amplification (LAMP) has provided a reliable substitute platform for the accurate detection of low copy number nucleic acids in the diagnosis of several viral diseases, including epidemics like Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS). At present, a cocktail of LAMP assay reagents along with reverse transcriptase enzyme (Reverse Transcription LAMP, RT-LAMP) can be a robust solution for the rapid and cost-effective diagnosis for COVID-19, particularly in developing, and low-income countries. In summary, the development of RT-LAMP based diagnostic tools in a paper/strip format or the integration of this method into a microfluidic platform such as a Lab-on-a-chip may revolutionize the concept of PoCT for COVID-19 diagnosis. This review discusses the principle, technology and past research underpinning the success for using this method for diagnosing MERS and SARS, in addition to ongoing research, and the prominent prospect of RT-LAMP in the context of COVID-19 diagnosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Augustine</LastName><ForeName>Robin</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, Doha 2713, Qatar.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biomedical Research Center (BRC), Qatar University, Doha PO Box 2713, Qatar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hasan</LastName><ForeName>Anwarul</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, Doha 2713, Qatar.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biomedical Research Center (BRC), Qatar University, Doha PO Box 2713, Qatar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Das</LastName><ForeName>Suvarthi</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0001-6094-2751</Identifier><AffiliationInfo><Affiliation>Department of Medicine, Stanford University Medical Center, Palo Alto, CA 94304, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahmed</LastName><ForeName>Rashid</ForeName><Initials>R</Initials><Identifier Source="ORCID">0000-0002-6512-769X</Identifier><AffiliationInfo><Affiliation>Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, Doha 2713, Qatar.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biomedical Research Center (BRC), Qatar University, Doha PO Box 2713, Qatar.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mori</LastName><ForeName>Yasuyoshi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Eiken Chemical Co., Ltd., Research and Development Division, Taito-ku 110-8408, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Notomi</LastName><ForeName>Tsugunori</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Eiken Chemical Co., Ltd., Research and Development Division, Taito-ku 110-8408, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kevadiya</LastName><ForeName>Bhavesh D</ForeName><Initials>BD</Initials><AffiliationInfo><Affiliation>Interventional Regenerative Medicine and Imaging Laboratory, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA 94304, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thakor</LastName><ForeName>Avnesh S</ForeName><Initials>AS</Initials><Identifier Source="ORCID">0000-0001-7395-0515</Identifier><AffiliationInfo><Affiliation>Interventional Regenerative Medicine and Imaging Laboratory, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA 94304, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>NPRP9-144-3-021</GrantID><Agency>Qatar National Research Fund</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D016454">Review</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Biology (Basel)</MedlineTA><NlmUniqueID>101587988</NlmUniqueID><ISSNLinking>2079-7737</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">COVID-19</Keyword><Keyword MajorTopicYN="Y">LAMP</Keyword><Keyword MajorTopicYN="Y">RT-LAMP</Keyword><Keyword MajorTopicYN="Y">SARS-CoV-2</Keyword><Keyword MajorTopicYN="Y">coronavirus</Keyword><Keyword MajorTopicYN="Y">point-of-care tests</Keyword><Keyword MajorTopicYN="Y">reverse transcription loop-mediated isothermal amplification</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>05</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>07</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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